single-base extension reaction Search Results


single base extension reaction  (Pyrosequencing Inc)
90
Pyrosequencing Inc single base extension reaction
<t>Reaction</t> chemistry of the 3 SNP genotyping systems tested. A: In solid-phase minisequencing, PCR is performed using biotinylated primers. The biotinylated PCR product is captured to a streptavidin (Strep)-coated, solid-phase (eg, microtitration plate), and denatured. The unbound strand is washed away from the reaction solution. A <t>single</t> nucleotide <t>extension</t> reaction using 3H deoxynucleotides is performed on the captured, single-stranded template. B: In <t>Pyrosequencing,</t> the PCR is performed using biotinylated primers and captured to a solid-phase (beads), similar to minisequencing. Yet the beads provide a much greater binding capacity than streptavidin-coated microtitration plates. The unbound strand is washed away. A four enzyme sequencing reaction, for a stretch of 4 to 5 consecutive nucleotides, is performed. Each nucleotide extension releases pyrophosphate. C: In the Tag-Array format, the PCR is performed using regular primers, but the denatured PCR product is captured by hybridization to oligonucleoties which have a Tag sequence as a tail. This tail sequence is complementary to a specific oligonucleotide Tag’s on the array. The primer extension reaction is performed using differently labeled fluorescent dideoxynucleotides.
Single Base Extension Reaction, supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/single base extension reaction/product/Pyrosequencing Inc
Average 90 stars, based on 1 article reviews
single base extension reaction - by Bioz Stars, 2026-03
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primers quantitative polymerase chain reaction (qpcr) single-base extension  (agena bioscience)
90
agena bioscience primers quantitative polymerase chain reaction (qpcr) single-base extension
<t>Reaction</t> chemistry of the 3 SNP genotyping systems tested. A: In solid-phase minisequencing, PCR is performed using biotinylated primers. The biotinylated PCR product is captured to a streptavidin (Strep)-coated, solid-phase (eg, microtitration plate), and denatured. The unbound strand is washed away from the reaction solution. A <t>single</t> nucleotide <t>extension</t> reaction using 3H deoxynucleotides is performed on the captured, single-stranded template. B: In <t>Pyrosequencing,</t> the PCR is performed using biotinylated primers and captured to a solid-phase (beads), similar to minisequencing. Yet the beads provide a much greater binding capacity than streptavidin-coated microtitration plates. The unbound strand is washed away. A four enzyme sequencing reaction, for a stretch of 4 to 5 consecutive nucleotides, is performed. Each nucleotide extension releases pyrophosphate. C: In the Tag-Array format, the PCR is performed using regular primers, but the denatured PCR product is captured by hybridization to oligonucleoties which have a Tag sequence as a tail. This tail sequence is complementary to a specific oligonucleotide Tag’s on the array. The primer extension reaction is performed using differently labeled fluorescent dideoxynucleotides.
Primers Quantitative Polymerase Chain Reaction (Qpcr) Single Base Extension, supplied by agena bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primers quantitative polymerase chain reaction (qpcr) single-base extension/product/agena bioscience
Average 90 stars, based on 1 article reviews
primers quantitative polymerase chain reaction (qpcr) single-base extension - by Bioz Stars, 2026-03
90/100 stars
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iselect single base extension reaction  (Illumina Inc)
90
Illumina Inc iselect single base extension reaction
<t>Reaction</t> chemistry of the 3 SNP genotyping systems tested. A: In solid-phase minisequencing, PCR is performed using biotinylated primers. The biotinylated PCR product is captured to a streptavidin (Strep)-coated, solid-phase (eg, microtitration plate), and denatured. The unbound strand is washed away from the reaction solution. A <t>single</t> nucleotide <t>extension</t> reaction using 3H deoxynucleotides is performed on the captured, single-stranded template. B: In <t>Pyrosequencing,</t> the PCR is performed using biotinylated primers and captured to a solid-phase (beads), similar to minisequencing. Yet the beads provide a much greater binding capacity than streptavidin-coated microtitration plates. The unbound strand is washed away. A four enzyme sequencing reaction, for a stretch of 4 to 5 consecutive nucleotides, is performed. Each nucleotide extension releases pyrophosphate. C: In the Tag-Array format, the PCR is performed using regular primers, but the denatured PCR product is captured by hybridization to oligonucleoties which have a Tag sequence as a tail. This tail sequence is complementary to a specific oligonucleotide Tag’s on the array. The primer extension reaction is performed using differently labeled fluorescent dideoxynucleotides.
Iselect Single Base Extension Reaction, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/iselect single base extension reaction/product/Illumina Inc
Average 90 stars, based on 1 article reviews
iselect single base extension reaction - by Bioz Stars, 2026-03
90/100 stars
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massextend (hme—single base extension) reaction  (Sequenom)
90
Sequenom massextend (hme—single base extension) reaction
<t>Reaction</t> chemistry of the 3 SNP genotyping systems tested. A: In solid-phase minisequencing, PCR is performed using biotinylated primers. The biotinylated PCR product is captured to a streptavidin (Strep)-coated, solid-phase (eg, microtitration plate), and denatured. The unbound strand is washed away from the reaction solution. A <t>single</t> nucleotide <t>extension</t> reaction using 3H deoxynucleotides is performed on the captured, single-stranded template. B: In <t>Pyrosequencing,</t> the PCR is performed using biotinylated primers and captured to a solid-phase (beads), similar to minisequencing. Yet the beads provide a much greater binding capacity than streptavidin-coated microtitration plates. The unbound strand is washed away. A four enzyme sequencing reaction, for a stretch of 4 to 5 consecutive nucleotides, is performed. Each nucleotide extension releases pyrophosphate. C: In the Tag-Array format, the PCR is performed using regular primers, but the denatured PCR product is captured by hybridization to oligonucleoties which have a Tag sequence as a tail. This tail sequence is complementary to a specific oligonucleotide Tag’s on the array. The primer extension reaction is performed using differently labeled fluorescent dideoxynucleotides.
Massextend (Hme—Single Base Extension) Reaction, supplied by Sequenom, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/massextend (hme—single base extension) reaction/product/Sequenom
Average 90 stars, based on 1 article reviews
massextend (hme—single base extension) reaction - by Bioz Stars, 2026-03
90/100 stars
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matrix-assisted laser desorption ionization-time-of-flight single-base extension reactions  (Sequenom)
90
Sequenom matrix-assisted laser desorption ionization-time-of-flight single-base extension reactions
<t>Reaction</t> chemistry of the 3 SNP genotyping systems tested. A: In solid-phase minisequencing, PCR is performed using biotinylated primers. The biotinylated PCR product is captured to a streptavidin (Strep)-coated, solid-phase (eg, microtitration plate), and denatured. The unbound strand is washed away from the reaction solution. A <t>single</t> nucleotide <t>extension</t> reaction using 3H deoxynucleotides is performed on the captured, single-stranded template. B: In <t>Pyrosequencing,</t> the PCR is performed using biotinylated primers and captured to a solid-phase (beads), similar to minisequencing. Yet the beads provide a much greater binding capacity than streptavidin-coated microtitration plates. The unbound strand is washed away. A four enzyme sequencing reaction, for a stretch of 4 to 5 consecutive nucleotides, is performed. Each nucleotide extension releases pyrophosphate. C: In the Tag-Array format, the PCR is performed using regular primers, but the denatured PCR product is captured by hybridization to oligonucleoties which have a Tag sequence as a tail. This tail sequence is complementary to a specific oligonucleotide Tag’s on the array. The primer extension reaction is performed using differently labeled fluorescent dideoxynucleotides.
Matrix Assisted Laser Desorption Ionization Time Of Flight Single Base Extension Reactions, supplied by Sequenom, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/matrix-assisted laser desorption ionization-time-of-flight single-base extension reactions/product/Sequenom
Average 90 stars, based on 1 article reviews
matrix-assisted laser desorption ionization-time-of-flight single-base extension reactions - by Bioz Stars, 2026-03
90/100 stars
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single-base extension polymerase chain reaction sequenom iplex-gold  (Sequenom)
90
Sequenom single-base extension polymerase chain reaction sequenom iplex-gold
<t>Reaction</t> chemistry of the 3 SNP genotyping systems tested. A: In solid-phase minisequencing, PCR is performed using biotinylated primers. The biotinylated PCR product is captured to a streptavidin (Strep)-coated, solid-phase (eg, microtitration plate), and denatured. The unbound strand is washed away from the reaction solution. A <t>single</t> nucleotide <t>extension</t> reaction using 3H deoxynucleotides is performed on the captured, single-stranded template. B: In <t>Pyrosequencing,</t> the PCR is performed using biotinylated primers and captured to a solid-phase (beads), similar to minisequencing. Yet the beads provide a much greater binding capacity than streptavidin-coated microtitration plates. The unbound strand is washed away. A four enzyme sequencing reaction, for a stretch of 4 to 5 consecutive nucleotides, is performed. Each nucleotide extension releases pyrophosphate. C: In the Tag-Array format, the PCR is performed using regular primers, but the denatured PCR product is captured by hybridization to oligonucleoties which have a Tag sequence as a tail. This tail sequence is complementary to a specific oligonucleotide Tag’s on the array. The primer extension reaction is performed using differently labeled fluorescent dideoxynucleotides.
Single Base Extension Polymerase Chain Reaction Sequenom Iplex Gold, supplied by Sequenom, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/single-base extension polymerase chain reaction sequenom iplex-gold/product/Sequenom
Average 90 stars, based on 1 article reviews
single-base extension polymerase chain reaction sequenom iplex-gold - by Bioz Stars, 2026-03
90/100 stars
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snp interrogation single-base extension reactions  (Sequenom)
90
Sequenom snp interrogation single-base extension reactions
<t>Reaction</t> chemistry of the 3 SNP genotyping systems tested. A: In solid-phase minisequencing, PCR is performed using biotinylated primers. The biotinylated PCR product is captured to a streptavidin (Strep)-coated, solid-phase (eg, microtitration plate), and denatured. The unbound strand is washed away from the reaction solution. A <t>single</t> nucleotide <t>extension</t> reaction using 3H deoxynucleotides is performed on the captured, single-stranded template. B: In <t>Pyrosequencing,</t> the PCR is performed using biotinylated primers and captured to a solid-phase (beads), similar to minisequencing. Yet the beads provide a much greater binding capacity than streptavidin-coated microtitration plates. The unbound strand is washed away. A four enzyme sequencing reaction, for a stretch of 4 to 5 consecutive nucleotides, is performed. Each nucleotide extension releases pyrophosphate. C: In the Tag-Array format, the PCR is performed using regular primers, but the denatured PCR product is captured by hybridization to oligonucleoties which have a Tag sequence as a tail. This tail sequence is complementary to a specific oligonucleotide Tag’s on the array. The primer extension reaction is performed using differently labeled fluorescent dideoxynucleotides.
Snp Interrogation Single Base Extension Reactions, supplied by Sequenom, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/snp interrogation single-base extension reactions/product/Sequenom
Average 90 stars, based on 1 article reviews
snp interrogation single-base extension reactions - by Bioz Stars, 2026-03
90/100 stars
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mass extend (single base extension) reaction  (Sequenom)
90
Sequenom mass extend (single base extension) reaction
<t>Reaction</t> chemistry of the 3 SNP genotyping systems tested. A: In solid-phase minisequencing, PCR is performed using biotinylated primers. The biotinylated PCR product is captured to a streptavidin (Strep)-coated, solid-phase (eg, microtitration plate), and denatured. The unbound strand is washed away from the reaction solution. A <t>single</t> nucleotide <t>extension</t> reaction using 3H deoxynucleotides is performed on the captured, single-stranded template. B: In <t>Pyrosequencing,</t> the PCR is performed using biotinylated primers and captured to a solid-phase (beads), similar to minisequencing. Yet the beads provide a much greater binding capacity than streptavidin-coated microtitration plates. The unbound strand is washed away. A four enzyme sequencing reaction, for a stretch of 4 to 5 consecutive nucleotides, is performed. Each nucleotide extension releases pyrophosphate. C: In the Tag-Array format, the PCR is performed using regular primers, but the denatured PCR product is captured by hybridization to oligonucleoties which have a Tag sequence as a tail. This tail sequence is complementary to a specific oligonucleotide Tag’s on the array. The primer extension reaction is performed using differently labeled fluorescent dideoxynucleotides.
Mass Extend (Single Base Extension) Reaction, supplied by Sequenom, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mass extend (single base extension) reaction/product/Sequenom
Average 90 stars, based on 1 article reviews
mass extend (single base extension) reaction - by Bioz Stars, 2026-03
90/100 stars
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sequencing primers for the multiplex single-base extension (sbe) reaction  (Qiagen)
90
Qiagen sequencing primers for the multiplex single-base extension (sbe) reaction
<t>Reaction</t> chemistry of the 3 SNP genotyping systems tested. A: In solid-phase minisequencing, PCR is performed using biotinylated primers. The biotinylated PCR product is captured to a streptavidin (Strep)-coated, solid-phase (eg, microtitration plate), and denatured. The unbound strand is washed away from the reaction solution. A <t>single</t> nucleotide <t>extension</t> reaction using 3H deoxynucleotides is performed on the captured, single-stranded template. B: In <t>Pyrosequencing,</t> the PCR is performed using biotinylated primers and captured to a solid-phase (beads), similar to minisequencing. Yet the beads provide a much greater binding capacity than streptavidin-coated microtitration plates. The unbound strand is washed away. A four enzyme sequencing reaction, for a stretch of 4 to 5 consecutive nucleotides, is performed. Each nucleotide extension releases pyrophosphate. C: In the Tag-Array format, the PCR is performed using regular primers, but the denatured PCR product is captured by hybridization to oligonucleoties which have a Tag sequence as a tail. This tail sequence is complementary to a specific oligonucleotide Tag’s on the array. The primer extension reaction is performed using differently labeled fluorescent dideoxynucleotides.
Sequencing Primers For The Multiplex Single Base Extension (Sbe) Reaction, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sequencing primers for the multiplex single-base extension (sbe) reaction/product/Qiagen
Average 90 stars, based on 1 article reviews
sequencing primers for the multiplex single-base extension (sbe) reaction - by Bioz Stars, 2026-03
90/100 stars
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primers for polymerase chain reaction and single-base extension  (agena bioscience)
90
agena bioscience primers for polymerase chain reaction and single-base extension
<t>Reaction</t> chemistry of the 3 SNP genotyping systems tested. A: In solid-phase minisequencing, PCR is performed using biotinylated primers. The biotinylated PCR product is captured to a streptavidin (Strep)-coated, solid-phase (eg, microtitration plate), and denatured. The unbound strand is washed away from the reaction solution. A <t>single</t> nucleotide <t>extension</t> reaction using 3H deoxynucleotides is performed on the captured, single-stranded template. B: In <t>Pyrosequencing,</t> the PCR is performed using biotinylated primers and captured to a solid-phase (beads), similar to minisequencing. Yet the beads provide a much greater binding capacity than streptavidin-coated microtitration plates. The unbound strand is washed away. A four enzyme sequencing reaction, for a stretch of 4 to 5 consecutive nucleotides, is performed. Each nucleotide extension releases pyrophosphate. C: In the Tag-Array format, the PCR is performed using regular primers, but the denatured PCR product is captured by hybridization to oligonucleoties which have a Tag sequence as a tail. This tail sequence is complementary to a specific oligonucleotide Tag’s on the array. The primer extension reaction is performed using differently labeled fluorescent dideoxynucleotides.
Primers For Polymerase Chain Reaction And Single Base Extension, supplied by agena bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primers for polymerase chain reaction and single-base extension/product/agena bioscience
Average 90 stars, based on 1 article reviews
primers for polymerase chain reaction and single-base extension - by Bioz Stars, 2026-03
90/100 stars
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Image Search Results


Reaction chemistry of the 3 SNP genotyping systems tested. A: In solid-phase minisequencing, PCR is performed using biotinylated primers. The biotinylated PCR product is captured to a streptavidin (Strep)-coated, solid-phase (eg, microtitration plate), and denatured. The unbound strand is washed away from the reaction solution. A single nucleotide extension reaction using 3H deoxynucleotides is performed on the captured, single-stranded template. B: In Pyrosequencing, the PCR is performed using biotinylated primers and captured to a solid-phase (beads), similar to minisequencing. Yet the beads provide a much greater binding capacity than streptavidin-coated microtitration plates. The unbound strand is washed away. A four enzyme sequencing reaction, for a stretch of 4 to 5 consecutive nucleotides, is performed. Each nucleotide extension releases pyrophosphate. C: In the Tag-Array format, the PCR is performed using regular primers, but the denatured PCR product is captured by hybridization to oligonucleoties which have a Tag sequence as a tail. This tail sequence is complementary to a specific oligonucleotide Tag’s on the array. The primer extension reaction is performed using differently labeled fluorescent dideoxynucleotides.

Journal:

Article Title: Comparison of GenFlex Tag Array and Pyrosequencing in SNP Genotyping

doi:

Figure Lengend Snippet: Reaction chemistry of the 3 SNP genotyping systems tested. A: In solid-phase minisequencing, PCR is performed using biotinylated primers. The biotinylated PCR product is captured to a streptavidin (Strep)-coated, solid-phase (eg, microtitration plate), and denatured. The unbound strand is washed away from the reaction solution. A single nucleotide extension reaction using 3H deoxynucleotides is performed on the captured, single-stranded template. B: In Pyrosequencing, the PCR is performed using biotinylated primers and captured to a solid-phase (beads), similar to minisequencing. Yet the beads provide a much greater binding capacity than streptavidin-coated microtitration plates. The unbound strand is washed away. A four enzyme sequencing reaction, for a stretch of 4 to 5 consecutive nucleotides, is performed. Each nucleotide extension releases pyrophosphate. C: In the Tag-Array format, the PCR is performed using regular primers, but the denatured PCR product is captured by hybridization to oligonucleoties which have a Tag sequence as a tail. This tail sequence is complementary to a specific oligonucleotide Tag’s on the array. The primer extension reaction is performed using differently labeled fluorescent dideoxynucleotides.

Article Snippet: Pyrosequencing Single Base Extension Reaction Pyrosequencing reactions were performed according to the manufacturer’s instructions with minor modifications (Pyrosequencing, Uppsala, Sweden).

Techniques: Binding Assay, Sequencing, Hybridization, Labeling

Comparison of Features of the Three SNP Genotyping Systems

Journal:

Article Title: Comparison of GenFlex Tag Array and Pyrosequencing in SNP Genotyping

doi:

Figure Lengend Snippet: Comparison of Features of the Three SNP Genotyping Systems

Article Snippet: Pyrosequencing Single Base Extension Reaction Pyrosequencing reactions were performed according to the manufacturer’s instructions with minor modifications (Pyrosequencing, Uppsala, Sweden).

Techniques: Comparison, Multiplex Assay, Microarray, Software